Meowmeow Posted April 13, 2020 Author Report Posted April 13, 2020 13 minutes ago, hotmaddy said: World War II lo alane chesaru edo rogam vasthe. It's proven that pooling blood samples work. However, pool chesthe Covid-19 virus detect avuthunda leda anedi naaku theliyadu. Experts are looking into that option though. If you are looking for the presence of an antibody using an ultra-senstive ELISA it makes sense. How will PCR work? No point. Quote
Naaperushiva Posted April 13, 2020 Report Posted April 13, 2020 20 minutes ago, Meowmeow said: If you are looking for the presence of an antibody using an ultra-senstive ELISA it makes sense. How will PCR work? No point. Why do you think it doesn't work can you elaborate....pooling anedhi used to take out the negative cases first... that way there wont be any bottleneck and the results kosam chaala rojulu wait cheyyakarledhu Quote
soodhilodaaram Posted April 13, 2020 Report Posted April 13, 2020 18 hours ago, Meowmeow said: It will give false negatives when samples are pooled. Increase testing personnel and testing equipment, don't pool samples. If you pool samples, Andhra is going to Assam. khurana testing emaina pickpocket testing aa oka type of pickpocketers ni oka prison cell divisions loki pool cheyadam la jebulu 10ge vedavalaki ilanti ideas ee vastaayi Quote
Meowmeow Posted April 13, 2020 Author Report Posted April 13, 2020 Just now, Naaperushiva said: Why do you think it doesn't work can you elaborate....pooling anedhi used to take out the negative cases first... that way there wont be any bottleneck and the results kosam chaala rojulu wait cheyyakarledhu By pooling 100 samples, you're diluting each sample by 100 times. Say five out of the 100 is positive, but PCR amplification after (1/20)th dilution will not be as effective as using the original concentration. So, it's more likely to come out as negative even though 5 people of the 100 are positive. Quote
bhaigan Posted April 13, 2020 Report Posted April 13, 2020 18 hours ago, Meowmeow said: It will give false negatives when samples are pooled. Increase testing personnel and testing equipment, don't pool samples. If you pool samples, Andhra is going to Assam. Agreed Quote
bhaigan Posted April 13, 2020 Report Posted April 13, 2020 Entire India is following pooling system not just jagan anna India lo Individual test kits vachinapudu appudu untadi asalu katha Quote
bhaigan Posted April 13, 2020 Report Posted April 13, 2020 18 hours ago, Meowmeow said: Also, look at the graph of Andhra, compare it with Kerala and Karnataka. Cases report cheyakapote vache graph laga obvious ga telustundi, when you compare it with Kerala and Karnataka. Kerala is the best performing state in entire India, always ahead with curve but lockdown helped AP a lot because tablighi event ki vellina valu max telugu valle ekkuva Quote
Naaperushiva Posted April 13, 2020 Report Posted April 13, 2020 6 minutes ago, Meowmeow said: By pooling 100 samples, you're diluting each sample by 100 times. Say five out of the 100 is positive, but PCR amplification after (1/20)th dilution will not be as effective as using the original concentration. So, it's more likely to come out as negative even though 5 people of the 100 are positive. There are many studies that states it otherwise not in just covid 19 related but also multiple other infections..That is one fast and effective methods used often in low resource countries.... There are multiple studies that states the contrary to your statement..But ofcourse every coin has two sides laaga every study varies too..But for now sampling is the best we can do ani naa abhiprayam Quote
Naaperushiva Posted April 13, 2020 Report Posted April 13, 2020 6 minutes ago, bhaigan said: Entire India is following pooling system not just jagan anna no other option bro..It's already too late ippudu testing kits personnel penchaali antey impossible manaki antha manchi infrastructure ledhu 1 Quote
bhaigan Posted April 13, 2020 Report Posted April 13, 2020 18 hours ago, Meowmeow said: Andhra statistics = China statistics India statistics = China statistics Quote
bhaigan Posted April 13, 2020 Report Posted April 13, 2020 Just now, Naaperushiva said: no other option bro..It's already too late ippudu testing kits personnel penchaali antey impossible manaki antha manchi infrastructure ledhu Exactly Quote
Naaperushiva Posted April 13, 2020 Report Posted April 13, 2020 anni times lo best ey kaavali antey kudharadhu..vunna vaatillo better solution tho povacchu... Quote
bhaigan Posted April 13, 2020 Report Posted April 13, 2020 @TrollBait appude cheppadu individual test kits vasthe Modi will be last PM of India ani but no one cared Quote
Meowmeow Posted April 13, 2020 Author Report Posted April 13, 2020 10 minutes ago, Naaperushiva said: There are many studies that states it otherwise not in just covid 19 related but also multiple other infections..That is one fast and effective methods used often in low resource countries.... There are multiple studies that states the contrary to your statement..But ofcourse every coin has two sides laaga every study varies too..But for now sampling is the best we can do ani naa abhiprayam Like I said, if you're testing antibody for ELISA it makes sense. Not for quantitative PCR especially for detection. Show me the studies if you have links to any. Quote
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